7个红掌品种产生愈伤组织的影响因素

采用正交试验,探讨在不同光照条件下MS大量元素、6-BA、2,4-D、NAA对7个红掌品种愈伤诱导的影响.结果表明:散射光照对红掌愈伤诱导有促进作用;时红掌愈伤诱导影响排序为:Ms大量元素>NAA>6-BA>2,4-D,大量元素的影响效果最明显,6-BA和2,4-D的影响效果差异较小.     

奶牛TNMD基因的克隆与原核表达

从奶牛组织中克隆Tenomodulin(TNMD)基因的cDNA序列,采用双酶切后连接表达载体pGEX-4T-1,转入大肠杆菌BL21中进行诱导表达。结果表明,TNMD基因的序列全长为957 bp,通过双酶切构建的表达载体pGEX-TNMD在BL21大肠杆菌中成功表达了分子量为59.68 kDa的融合蛋白。     

菜薹蛋白精氨酸甲基转移酶基因BrcuPRMT5的克隆及表达分析

以‘油青49’和‘油青甜菜薹80’菜薹茎尖为材料,采用RT-PCR和RACE技术克隆获得组蛋白甲基转移酶基因Brcu PRMT5的全长cDNA和gDNA序列。BrcuPRMT5 cDNA序列全长为2 117 bp,其中完整开放阅读框为1 929 bp,编码642个氨基酸,相对分子量为71.55 kD,理论等电点(p I)为5.87;多序列比对结果表明,Brcu PRMT5编码的氨基酸序列含有高等植物PRMT5基因1个高度保守的结构域;系统发育分析结果显示与大白菜、油菜及甘蓝的亲缘关系最近;亚细胞定位软件分析得知,Brcu PRMT5蛋白无跨膜区域,可能定位于线粒体中;对应的gDNA全长为4 151 bp,含有23个外显子和22个内含子,最长的外显子长度为140 bp,内含子的长度范围为50~150 bp。利用半定量RT-PCR和实时荧光定量PCR技术分析基因的表达,BrcuPRMT5在菜薹不同组织中均有表达,其中在花中表达量最高,叶次之,根中最低;BrcuPRMT5从苗期至完全抽薹开花期的表达量呈现上升趋势。BrcuPRMT5在菜薹花发育过程中可能起一定的调控作用。     

如何提高奥氏气体分析仪检测粮堆中CO_2和O_2浓度的准确度

介绍了检测粮堆中CO_2和O_2气体浓度所用奥氏气体分析仪的构造、原理、操作,提出了提高奥氏气体分析仪检测准确度的方法建议。     

绿皮苦瓜新品种冠春5号的选育

冠春5号是以高代自交系327作母本,以自交系186作父本配制而成的苦瓜一代杂种。植株生长势旺盛,早熟,从播种至始收82 d(天)。主侧蔓均可结果,主蔓第1雌花节位为第7~8节。瓜长棒形,瓜长25~35 cm,横径5~7 cm,肉厚0.9~1.0 cm,单瓜质量420 g左右。果表皮浅绿色,点状突起与短条状突起相间瘤状物,尾部钝,果肉脆,苦味淡。早春栽培一般每667 m~2产量约3 000 kg,田间对白粉病和疫病的抗性优于对照新农村油绿苦瓜。适宜四川、重庆等地种植。     

(台中科技大学 应用中文系，台中 台湾404)

庄子其人，个性好自由不受拘束，思想超乎常人想像之外，笔调流畅，时间、空间及世人的看法所不能拘束，生死富贵也不能束缚他。《庄子》其书，可与《老子》同样尊贵，同样成为道家学术思想之主流，主导了魏晋玄学的哲学思路，影响了佛教中国禅的形成，也成为宋明理学的重要理论基础。而他的让心灵自由（心斋坐忘），更是道教上清斋醮最高修炼法门。庄子其人其书，对后世的影响，可以说是至深且广。但庄子其人，有宋人、魏人之争；《庄子》其书，则有真假及篇章多少之辩。今以出土文物及现存史料看来，庄子应为宋人，《庄子》书应为庄子所著，而内、外、杂三篇的形成，则当在魏晋南北朝时，系沿袭晋·郭象之归类而来。     

Evaluation on Tolerance of Montmorillonite as a Mycotoxin Adsorbent

In order to study the tolerance dose of Kunming mice to montmorillonite as a mycotoxin adsorbent in diets,the experiment selected 60 healthy male Kunming mice with initial average body weight of (17±0.12)g,randomly divided them into 5 treatments with two replicates per group and 6 mice per replicate.Five experimental diets were added to 0 (control group),0.5%,1%,2% and 5% montmorillonite,respectively.After 30 days,the growth performance,nutrients apparent digestibility,serum biochemical indices and internal organization were tested to determine the tolerance dose of montmorillonite for Kunming mice.The results showed that the ADFI was significantly increased in 0.5% group (P< 0.05),while there were no significant differences in ADFI and ADG between 1% group and control group (P> 0.05),then the ADFI was significantly decreased with the increasing of montmorillonite supplemental level (P< 0.05).The ADG in 2% group had no significant difference when compared with the control group (P> 0.05),while significantly lower than that in 0.5% and 1% groups (P< 0.05).The apparent digestibility of DM,CP and EE showed no significant effect at any level of montmorillonite (P> 0.05).Serum ALT,AST activities and MDA content were gradually increased with the montmorillonite supplemental level increasing,and that in 1%,2% and 5% groups were significantly higher than that in control group (P< 0.05),but there were no significant differences between 0.5% group and control group (P> 0.05).Tissue sections showed that mice livers were damaged in varying degrees when the montmorillonite supplemental level was 5%.In conclusion,based on a comprehensive consideration of all indexes,it could be seen that the tolerance dose of the tested montmorillonite for Kunming mice was between 0.5% and 2% when mycotoxin contents in diets were in security range.     

设施韭菜安全生产关键技术集成

生产绿色、安全、放心韭菜，除了应用优质品种、实施绿色韭菜安全施肥技术外，推广绿色韭菜韭蛆防控新技术是关键。     

论生态景观吸引力空间异化模型的应用——以江苏环太湖国际旅游区规划为例

以江苏环太湖国际旅游区规划为实验对象,重点探讨生态景观吸引力空间异化过程,即从要素磁场分化开始,经由同质景观间的引力异化,导致同类景观片区间的引力空间异化,最终推导出另一种"同质相斥、异质相吸"的新型生态景观吸引力空间结构。